| Title | The study of enantioselectivity of all regioisomers of mono-carboxymethyl-β-cyclodextrin used as chiral selectors in CE |
| Publication Type | Journal Article |
| Year of Publication | 2013 |
| Authors | Navrátilová K, Řezanka P, Řezanka M, Sýkora D, Jindřich J, Král V |
| Journal | Journal of Separation Science |
| Volume | 36 |
| Issue | 7 |
| Pagination | 1270 - 1274 |
| Date Published | 2013/// |
| ISBN Number | 1615-9314 |
| Keywords | CE, Chiral separation, duplicate, ENANTIOSELECTIVITY, Monosubstituted carboxymethyl-β-cyclodextrin, Regioisomer |
| Abstract | This work documents the influence of the position of single carboxymethyl group on the β-cyclodextrin skeleton on the enantioselectivity. These synthesized monosubstituted carboxymethyl cyclodextrin (CD) derivatives, native β-cyclodextrin, and commercially available carboxymethyl-β-cyclodextrin with degree of substitution approximately 3 were used as additives into the BGE consisting of phosphate buffer at 20 mmol/L concentration, pH 2.5, and several biologically significant low-molecular-mass chiral compounds were enantioseparated by CE. The results indicate that different substituent location on β-cyclodextrin skeleton has a significant influence on the enantioseparation of the investigated enantiomers. The enantioselectivity of 2I-O-regioisomer was better than with native β-cyclodextrin. Comparable results to native β-cyclodextrin were obtained for 6I-O- regioisomer and the enantioselectivity of 3I-O-regioisomer was even worse than with native β-cyclodextrin. Commercially available derivative of CD provides better resolutions than the monosubstituted carboxymethyl CD derivatives for most of the investigated analytes. |
| URL | http://onlinelibrary.wiley.com/doi/10.1002/jssc.201201144/abstract |
| Short Title | J. Sep. Sci. |
