Determination of cyclodextrins and their derivatives by capillary electrophoresis with indirect UV and conductivity detection

TitleDetermination of cyclodextrins and their derivatives by capillary electrophoresis with indirect UV and conductivity detection
Publication TypeJournal Article
Year of Publication2001
AuthorsPumera M, Jelinek I, Jindrich J
JournalFresenius Journal of Analytical Chemistry
Volume369
Issue7-8
Pagination666 - 669
Date Published2001/04//
ISBN Number0937-0633
AbstractA fast and simple capillary electrophoretic method suitable for the determination of native alpha-, beta-, gamma -cyclodextrins their randomly substituted tert-butyl derivatives (average degree of substitution 3.8 - 4.4), heptakis (2,6-di-O-methyl)- and heptakis (2,3,6-tri-O-methyl)-beta -cyclodextrin was developed. Naphthyl-2-sulfonic acid (2-NSA). 3-iodobenzoic acid (3-IBA) and (1S)-1-phenylethylamine (PHEA) were tested as selective complex forming and UV absorbing background electrolyte additives. The composition of optimized background electrolyte for the separation of uncharged cyclodextrins and their derivatives tvas: 15 mM 3-iodobenzoic acid titrated with tris[hydroxymethyl]aminomethane to pH 8.0, 5% (v/v) of acetonitrile. A complete resolution of mono-2-O-, mono-3-O- and mono-4-O-carboxymethyl-beta -cyclodextrin regioisomers was achieved in the optimized background electrolyte system: 40 mM PHEA titrated with 2-[N-morpholino]ethanesulfonic acid to pH 5.6. In addition to indirect UV detection a contactless conductometric detector was successfully utilized.
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Short TitleFresenius J. Anal. Chem.
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